Monitoring Cancer-Linked HPV Through Wastewater Analysis

Fri 23rd May, 2025

Recent research from Uruguay has demonstrated the potential of wastewater analysis in tracking genotypes of human papillomavirus (HPV), a primary contributor to cervical cancer. The findings, published in the journal Food and Environmental Virology, highlight wastewater monitoring as an innovative tool for understanding disease prevalence, particularly in low- and middle-income countries where epidemiological data is often lacking.

HPV is responsible for 99% of cervical cancer cases, which disproportionately affects women in lower-income regions. The research team, led by scientists from the University of the Republic's Molecular Virology Laboratory, has emphasized that environmental monitoring can provide crucial insights into public health trends even before clinical cases are reported.

During the study conducted in Salto, a city with a population of approximately 114,000, researchers collected monthly wastewater samples from the city's sewage system over the course of a year. Their genetic analysis revealed the presence of HPV in 75% of the samples, identifying a total of 28 different genotypes. Among these, eight were classified as high-risk for cervical cancer, including the notorious genotypes 16 and 18, while six were low-risk types associated with genital warts.

In addition to analyzing wastewater, the researchers also conducted clinical tests on 140 Pap smear samples, finding HPV presence in 45% of these tests. A comparison between the two sample types showed a significant overlap, with 88% of detected genotypes found in both clinical and wastewater samples. This correlation suggests that wastewater-based epidemiology could be an effective method for monitoring HPV prevalence.

Experts believe that the results of this study could catalyze further epidemiological research and enhance prevention strategies for cervical cancer and other HPV-related cancers. The presence of HPV in wastewater is attributed to the virus being expelled through bodily fluids such as urine, feces, and skin washing, making wastewater an informative medium for health surveillance.

Despite the availability of HPV vaccines offered free of charge in Uruguay to individuals aged 11 to 26, vaccination coverage remains suboptimal. Current statistics indicate that around 65% of girls and 55% of boys under 15 have received the first dose of the vaccine. The researchers hope that ongoing vaccination campaigns will lead to a reduction in the prevalence of high-risk HPV genotypes in the future.

As wastewater monitoring becomes increasingly recognized for its utility in public health surveillance, the study underscores its importance in the aftermath of the COVID-19 pandemic. Prior to this, wastewater analysis primarily focused on fecal-oral viruses, but the current study illustrates the potential for broader applications in tracking various pathogens, including those linked to cancer.


More Quick Read Articles »