New Rapid Test Offers Affordable Detection for Trichomoniasis in 15 Minutes

Trichomoniasis, caused by the parasite Trichomonas vaginalis, ranks among the most prevalent sexually transmitted infections globally. This condition often goes undetected due to its asymptomatic nature, complicating timely diagnosis and treatment. Early detection is crucial, as untreated infections can lead to severe reproductive health issues, increase susceptibility to HIV, and cause genital inflammation.

In response to this healthcare challenge, researchers from the Universitat Rovira i Virgili (URV) have developed an innovative and cost-effective testing method that can identify the parasite responsible for trichomoniasis in just 15 minutes. The findings of this study have been published in the journal Sensors & Diagnostics.

The new test, known as the Aptamer-based Lateral Flow Assay (ALFA), is priced at under one euro, making it an accessible option for public health initiatives, particularly in regions with limited healthcare resources. The ALFA test leverages advanced technology based on DNA aptamers, which are short strands of nucleic acids designed to bind specifically to target molecules. This dual-aptamer system includes one aptamer immobilized on a nitrocellulose membrane, while another is attached to gold nanoparticles, facilitating a visual detection signal.

According to the researchers, this innovative approach has not only reduced production costs but has also enhanced the stability of the test, ensuring that its components maintain their efficacy for up to a year at room temperature (22 ºC). This characteristic eliminates the need for refrigeration or special storage conditions, allowing for broader distribution in resource-limited settings.

Conducting the test is straightforward, requiring only three steps from sample collection to result interpretation. The results are available in approximately 15 minutes. In preliminary studies utilizing clinical samples, the outcomes from the ALFA test matched perfectly with those obtained through traditional diagnostic methods, validating its reliability.

The research team plans to further their investigations by adapting the ALFA methodology to detect additional pathogens, potentially expanding its applicability in the medical field.